Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 Jan 1;61(Pt 1):87-9.
doi: 10.1107/S1744309104029197. Epub 2004 Dec 2.

Crystallization and preliminary X-ray diffraction analysis of a lectin from Canavalia maritima seeds

Carlos Alberto de Almeida Gadelha  1 , Frederico Bruno Mendes Batista Moreno, Tatiane Santi-Gadelha, João Batista Cajazeiras, Bruno Anderson M da Rocha, Joane Kathelen Rodrigues Rustiguel, Beatriz Tupinamba Freitas, Fernanda Canduri, Plínio Delatorre, Walter Filgueira de Azevedo Jr, Benildo S Cavada
Affiliations

Crystallization and preliminary X-ray diffraction analysis of a lectin from Canavalia maritima seeds

Carlos Alberto de Almeida Gadelha et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. .

Abstract

A lectin from Canavalia maritima seeds (ConM) was purified and submitted to crystallization experiments. The best crystals were obtained using the vapour-diffusion method at a constant temperature of 293 K and grew in 7 d. A complete structural data set was collected to 2.1 A resolution using a synchrotron-radiation source. The ConM crystal belongs to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 67.15, b = 70.90, c = 97.37 A. A molecular-replacement search found a solution with a correlation coefficient of 69.2% and an R factor of 42.5%. Crystallographic refinement is under way.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Native crystal of the lectin from C. maritima seeds.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Alencar, N. M. N., Teixeira, E. H., Assreuy, A. M., Cavada, B. S., Flores, C. A. & Ribeiro, R. A. (1999). Mediators Inflamm.8, 107–113. - PMC - PubMed
    1. Andrade, J. L., Arruda, S., Barbosa, T., Paim, L., Ramos, M. V., Cavada, B. S. & Barral-Netto, M. (1999). Cell. Immunol.194, 98–102. - PubMed
    1. Assreuy, A. M., Martins, G. J., Moreira, M. E. F., Brito, G. A. C., Cavada, B. S., Ribeiro, R. A. & Flores, C. A. (1999). J. Urol.161, 1988–1993. - PubMed
    1. Barbosa, T., Arruda, S., Cavada, B. S., Grangeiro, T. B., Freitas, L. A. R. & Barral-Netto, M. (2001). Mem. Inst. Oswaldo Cruz, 96, 673–678. - PubMed
    1. Barral-Neto, M., Santos, S. B., Barral, A., Moreira, L. I. M., Santos, C. F., Moreira, R. A., Oliveira, J. T. A. & Cavada, B. S. (1992). Immunol. Invest.21, 297–303. - PubMed

Publication types