Crystallization and preliminary X-ray studies of a non-haemorrhagic fibrin(ogen)olytic metalloproteinase from the venom of Agkistrodon acutus

Abstract

A non-haemorrhagic fibrin(ogen)olytic metalloproteinase from the venom of Agkistrodon acutus has been crystallized by the hanging-drop method. The crystals belong to space group P3(1)21, with unit-cell parameters a = b = 80.57, c = 66.77 A and one molecule in the asymmetric unit. X-ray diffraction data were collected to 1.86 A resolution.

Figure 1

Crystals of FII. (a) Crystals grown in 1.4 M trisodium citrate, 0.1 M Na HEPES pH 7.5 (resolution 1.86 Å); (b) crystals grown in 2.0 M ammonium sulfate, 0.1 M sodium citrate pH 5.6, 0.2 M potassium/sodium tartrate (resolution 4.0 Å); (c) crystals grown in 30%(w/v) PEG 4000, 0.1 M Tris–HCl pH 8.5, 0.2 M lithium sulfate (resolution 3.5 Å); (d) crystals grown in 30%(w/v) PEG 8000, 0.1 M sodium cacodylate pH 6.5, 0.2 M ammonium sulfate (resolution 2.8 Å).

Figure 2

Typical diffraction pattern for a crystal grown in 1.4 M trisodium citrate, 0.1 M Na HEPES pH 7.5. The contents of the rectangle in the top left image are magnified in the bottom right part of the figure.