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. 2005 Jul 1;61(Pt 7):669-72.
doi: 10.1107/S1744309105017549. Epub 2005 Jun 15.

Purification, crystallization and preliminary X-ray diffraction studies of the three components of the toluene 2,3-dioxygenase enzyme system

Kyoung Lee  1 , Rosmarie Friemann, Juan V Parales, David T Gibson, S Ramaswamy
Affiliations

Purification, crystallization and preliminary X-ray diffraction studies of the three components of the toluene 2,3-dioxygenase enzyme system

Kyoung Lee et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. .

Erratum in

  • Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Apr 1;62(Pt 4):422

Abstract

Pseudomonas putida F1 can grow with toluene as its sole source of carbon and energy. The initial reaction of the degradation of toluene is catalyzed by a three-component toluene dioxygenase enzyme system consisting of a reductase (ReductaseTOL), a ferredoxin (FerredoxinTOL) and a Rieske non-heme iron dioxygenase (OxygenaseTOL). The three components and the apoenzyme of the dioxygenase (apo-OxygenaseTOL) were overexpressed, purified and crystallized. ReductaseTOL diffracts to 1.8 A and belongs to space group P4(1)2(1)2, with unit-cell parameters a = b = 77.1, c = 156.3 A. Ferredoxin(TOL) diffracts to 1.2 A and belongs to space group P2(1), with unit-cell parameters a = 30.5, b = 52.0, c = 30.95 A, beta = 113.7 degrees. Apo-OxygenaseTOL and OxygenaseTOL diffract to 3.2 A and belong to space group P4(3)32, with unit-cell parameters a = 235.9 A and a = 234.5 A, respectively.

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Figures

Figure 1
Figure 1
Dihydroxylation of toluene to cis-toluene dihydrodiol catalyzed by the three-component toluene dioxygenase enzyme system.
Figure 2
Figure 2
Photographs of crystals of ReductaseTOL (a), FerredoxinTOL (b) and OxygenaseTOL (c).
See this image and copyright information in PMC

References

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