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. 2005 Jul 1;61(Pt 7):712-4.
doi: 10.1107/S1744309105019093. Epub 2005 Jun 30.

Crystallization and preliminary X-ray diffraction study of BchU, a methyltransferase from Chlorobium tepidum involved in bacteriochlorophyll c biosynthesis

Jiro Harada  1 , Kei Wada, Hitomi Yamaguchi, Hirozo Oh-oka, Hitoshi Tamiaki, Keiichi Fukuyama
Affiliations

Crystallization and preliminary X-ray diffraction study of BchU, a methyltransferase from Chlorobium tepidum involved in bacteriochlorophyll c biosynthesis

Jiro Harada et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. .

Abstract

The S-adenosylmethionine-dependent methyltransferase BchU is an enzyme involved in the bacteriochlorophyll c biosynthetic pathway and catalyzes methylation at the C-20 position of the chlorin moiety. Recombinant Chlorobium tepidum BchU overproduced in Escherichia coli was purified and crystallized by the hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant. The crystals belonged to the hexagonal space group P6(1)22 or P6(5)22, with unit-cell parameters a = b = 81.5, c = 250.7 A. A native data set was collected to 2.27 A resolution using synchrotron radiation at SPring-8.

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Figures

Figure 1
Figure 1
Molecular structures of BChls c and d and BChlids c and d (left) and Zn-BChlids c/d (right). The 31 stereochemistry is R or S.
Figure 2
Figure 2
A typical crystal of the His6-BchU from C. tepidum. The dimensions are approximately 0.1 × 0.1 × 0.4 mm.
Figure 3
Figure 3
Diffraction pattern of the His6-BchU crystal. The resolution of the spot indicated by the arrow is 2.10 Å.
See this image and copyright information in PMC

References

    1. Blankenship, R. E. & Matsuura, K. (2003). Light-Harvesting Antennas in Photosynthesis, edited by B. R. Green & W. W. Parson, pp. 195–217. Dordrecht: Kluwer Academic Publishers.
    1. Blankenship, R. E., Olson, J. M. & Miller, M. (1995). Anoxygenic Photosynthetic Bacteria, edited by R. E. Blankenship, M. T. Madigan & C. E. Bauer, pp. 399–435. Dordrecht: Kluwer Academic Publishers.
    1. Eisen, J. A. et al. (2002). Proc. Natl Acad. Sci. USA, 99, 9509–9514. - PubMed
    1. Frigaard, N. U. & Bryant, D. A. (2004). Arch. Microbiol.182, 265–276. - PubMed
    1. Frigaard, N. U., Chew, A. G. M., Li, H., Maresca, J. A. & Bryant, D. A. (2003). Photosynth. Res.78, 93–117. - PubMed

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