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. 2005 Oct 1;61(Pt 10):906-9.
doi: 10.1107/S1744309105027995. Epub 2005 Sep 30.

Crystallization and preliminary X-ray crystallographic analysis of Sulfolobus solfataricus thioredoxin reductase

Alessia Ruggiero  1 , Maria Rosaria Ruocco, Pasquale Grimaldi, Paolo Arcari, Mariorosario Masullo, Adriana Zagari, Luigi Vitagliano
Affiliations

Crystallization and preliminary X-ray crystallographic analysis of Sulfolobus solfataricus thioredoxin reductase

Alessia Ruggiero et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. .

Abstract

A thermostable thioredoxin reductase isolated from Sulfolobus solfataricus (SsTrxR) has been successfully crystallized in the absence and in the presence of NADP. Two different crystal forms have been obtained. Crystals of the form that yields higher resolution data (1.8 A) belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 76.77, b = 120.68, c = 126.85 A. The structure of the enzyme has been solved by MAD methods using the anomalous signal from the Se atoms of selenomethionine-labelled SsTrxR.

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Figures

Figure 1
Figure 1
Images of typical SsTrxR crystals: (a) crystal form I, (b) crystal form II.
Figure 2
Figure 2
Diffraction pattern of a typical SsTrxR crystal (form I). Diffraction data are detectable to 1.8 Å resolution.
See this image and copyright information in PMC

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