An accurate and reproducible method for proteome profiling of the effects of salt stress in the rice leaf lamina
- PMID: 16513811
- DOI: 10.1093/jxb/erj134
An accurate and reproducible method for proteome profiling of the effects of salt stress in the rice leaf lamina
Abstract
Proteomic analysis of any biological system by two-dimensional gel electrophoresis (2-DE) requires high resolution and high reproducibility. The results presented here demonstrate the reproducible and accurate separation of rice (Oryza sativa L.) proteins using improved procedures for high resolution 2-DE, which were adapted for the separation of rice lamina proteins. Validation of this system was achieved by measuring the effects of sample preparation and biological variation on the coefficient of variation (CV) for replicate spots. The majority of experimental variation was shown to be introduced by the 2-DE technique (CV 0.26). Analysis of biological variation indicated that approximately 93-95% of spots were within a CV of 0.7. This provided a threshold value from which valid differences in expression between experimental groups could be screened. This system was then utilized for the proteomic analysis of short- and long-term salt-stress-responsive proteins in the rice leaf lamina. Analysis resulted in the separation of approximately 2500 protein species of which 32 were observed to be significantly regulated by salinity; so far 11 of these proteins have been identified by tandem mass spectrometry. An increase in eight proteins, including RuBisCO activase and ferritin, occurred by 24 h of exposure to sodium chloride (50 mM) and continued to increase during the following 6 d. Only one protein, a putative phosphoglycerate kinase, was found to increase in expression within 24 h and did not increase over a longer period of exposure to salt. There were also proteins that showed no change 24 h after exposure to salt, but had increased (superoxide dismutase) or decreased (S-adenosyl-L-methionine synthetase) after 7 d salt treatment.
Similar articles
-
A hydroponic rice seedling culture model system for investigating proteome of salt stress in rice leaf.Electrophoresis. 2005 Dec;26(23):4521-39. doi: 10.1002/elps.200500334. Electrophoresis. 2005. PMID: 16315177
-
A proteomic approach to analyze salt-responsive proteins in rice leaf sheath.Proteomics. 2004 Jul;4(7):2072-81. doi: 10.1002/pmic.200300741. Proteomics. 2004. PMID: 15221768
-
[A two-dimensional electrophoresis protocol suitable for proteomic study of rice leaves].Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006 Apr;32(2):252-6. Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006. PMID: 16622327 Chinese.
-
Rice proteomics: ending phase I and the beginning of phase II.Proteomics. 2009 Feb;9(4):935-63. doi: 10.1002/pmic.200800594. Proteomics. 2009. PMID: 19212951 Review.
-
Rejuvenating rice proteomics: facts, challenges, and visions.Proteomics. 2006 Oct;6(20):5549-76. doi: 10.1002/pmic.200600233. Proteomics. 2006. PMID: 16991195 Review.
Cited by
-
Physiological and proteomic responses of diploid and tetraploid black locust (Robinia pseudoacacia L.) subjected to salt stress.Int J Mol Sci. 2013 Oct 14;14(10):20299-325. doi: 10.3390/ijms141020299. Int J Mol Sci. 2013. PMID: 24129170 Free PMC article.
-
Physiological and proteomic analysis in chloroplasts of Solanum lycopersicum L. under silicon efficiency and salinity stress.Int J Mol Sci. 2014 Nov 26;15(12):21803-24. doi: 10.3390/ijms151221803. Int J Mol Sci. 2014. PMID: 25431925 Free PMC article.
-
Influence of salicylic acid on rubisco and rubisco activase in tobacco plant grown under sodium chloride in vitro.Saudi J Biol Sci. 2014 Nov;21(5):417-26. doi: 10.1016/j.sjbs.2014.04.002. Epub 2014 May 2. Saudi J Biol Sci. 2014. PMID: 25313276 Free PMC article.
-
Salinity-induced inhibition of growth in the aquatic pteridophyte Azolla microphylla primarily involves inhibition of photosynthetic components and signaling molecules as revealed by proteome analysis.Protoplasma. 2017 Jan;254(1):303-313. doi: 10.1007/s00709-016-0946-2. Epub 2016 Feb 2. Protoplasma. 2017. PMID: 26837223
-
Proteomic identification of differentially expressed proteins between male and female plants in Pistacia chinensis.PLoS One. 2013 May 17;8(5):e64276. doi: 10.1371/journal.pone.0064276. Print 2013. PLoS One. 2013. PMID: 23691188 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources