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Comparative Study
. 1991 Sep;65(9):4670-80.
doi: 10.1128/JVI.65.9.4670-4680.1991.

Characterization of the DNA polymerase gene of human herpesvirus 6

I A Teo  1 , B E Griffin, M D Jones
Affiliations
Comparative Study

Characterization of the DNA polymerase gene of human herpesvirus 6

I A Teo et al. J Virol. 1991 Sep.

Abstract

The construction of a recombinant bacteriophage lambda library containing overlapping clones covering 155 kbp of the 161-kbp genome of the Ugandan U1102 isolate of human herpesvirus 6 (HHV-6) is described. The use of degenerate-primer polymerase chain reaction allowed the isolation of a DNA probe for the DNA polymerase gene of HHV-6, which was subsequently used to isolate and position the pol gene on the physical map of the viral genome. A 4.4-kbp EcoRI DNA restriction fragment containing the pol gene was isolated and sequenced. The open reading frames flanking the pol gene code for the HHV-6 glycoprotein B gene and the human cytomegalovirus UL53 homolog. This arrangement is different from that seen in the alpha and gamma herpesvirus families, lending further support to the notion that HHV-6 is a member of the beta herpesvirus group.

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References

    1. Proc Natl Acad Sci U S A. 1990 Oct;87(20):7963-7 - PubMed
    1. Virology. 1988 Nov;167(1):207-25 - PubMed
    1. Oncogene. 1990 Sep;5(9):1365-70 - PubMed
    1. J Mol Biol. 1987 Nov 20;198(2):327-37 - PubMed
    1. J Virol. 1988 Dec;62(12):4493-8 - PubMed

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