Brevundimonas kwangchunensis sp. nov., isolated from an alkaline soil in Korea

Abstract

Two Gram-negative, rod-shaped bacterial strains, KSL-102T and KSL-110, were isolated from an alkaline soil in Korea, and their taxonomic positions were investigated by use of a polyphasic study. The two strains grew optimally at pH 7.0-8.0 and 30 degrees C without NaCl. They contained Q-10 as the predominant ubiquinone. The major fatty acids were C(18:1)omega7c and C(16:0) on trypticase soy agar, but 11-methyl C(18:1)omega7c was also a major component when the two strains were cultivated on LMG medium no. 221. Their DNA G + C contents were 68.4-68.7 mol%. Strains KSL-102T and KSL-110 exhibited three nucleotide differences in their 16S rRNA gene sequences and a mean DNA-DNA relatedness value of 85 %. Phylogenetic trees based on 16S rRNA gene sequences showed that the two strains fell within the evolutionary radiation encompassed by the genus Brevundimonas. Levels of 16S rRNA gene sequence similarity between the two strains and the type strains of recognized Brevundimonas species ranged from 96.3 to 98.4%. DNA-DNA relatedness levels between the two strains and recognized Brevundimonas species were 8-21%. On the basis of phenotypic, phylogenetic and genetic data, strains KSL-102T and KSL-110 were classified in the genus Brevundimonas as members of a novel species, for which the name Brevundimonas kwangchunensis sp. nov. is proposed. The type strain is KSL-102T (= KCTC 12380T = DSM 17033T).