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. 1991 Aug 15;88(16):7351-5.
doi: 10.1073/pnas.88.16.7351.

The N-end rule is mediated by the UBC2(RAD6) ubiquitin-conjugating enzyme

R J Dohmen  1 , K Madura, B Bartel, A Varshavsky
Affiliations

The N-end rule is mediated by the UBC2(RAD6) ubiquitin-conjugating enzyme

R J Dohmen et al. Proc Natl Acad Sci U S A. .

Abstract

The N-end rule relates the in vivo half-life of a protein to the identity of its amino-terminal residue. Distinct versions of the N-end rule operate in all organisms examined, from mammals to bacteria. We show that UBC2(RAD6), one of at least seven ubiquitin-conjugating enzymes in the yeast Saccharomyces cerevisiae, is essential for multiubiquitination and degradation of the N-end rule substrates. We also show that UBC2 is physically associated with UBR1, the recognition component of the N-end rule pathway. These results indicate that some of the UBC2 functions, which include DNA repair, induced mutagenesis, sporulation, and regulation of retrotransposition, are mediated by protein degradation via the N-end rule pathway.

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References

    1. J Biol Chem. 1988 Feb 25;263(6):2693-8 - PubMed
    1. Microbiol Rev. 1988 Mar;52(1):70-102 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 Jan;82(1):168-72 - PubMed
    1. J Biol Chem. 1990 Mar 25;265(9):4789-92 - PubMed
    1. Mol Cell Biol. 1990 Mar;10(3):1017-22 - PubMed

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