Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2006 Mar;44(3):688-92.
doi: 10.1128/JCM.44.3.688-692.2006.

Multicenter laboratory validation of the BACTEC MGIT 960 technique for testing susceptibilities of Mycobacterium tuberculosis to classical second-line drugs and newer antimicrobials

Sabine Rüsch-Gerdes  1 , Gaby E Pfyffer, Manuel Casal, Maureen Chadwick, Salman Siddiqi
Affiliations
Comparative Study

Multicenter laboratory validation of the BACTEC MGIT 960 technique for testing susceptibilities of Mycobacterium tuberculosis to classical second-line drugs and newer antimicrobials

Sabine Rüsch-Gerdes et al. J Clin Microbiol. 2006 Mar.

Abstract

The BACTEC MGIT 960 system, a fully automated, nonradiometric, noninvasive system for detection and drug susceptibility testing of mycobacteria, was evaluated for the ability to test susceptibilities to second-line drugs. In this study, which was carried out in three phases (phase I, mostly susceptible strains; phase II, mostly resistant strains; phase III, final testing of the optimal drug concentrations found in phases I and II), we established the critical concentrations for seven drugs to be tested in the BACTEC MGIT 960 system compared to the BACTEC 460TB system. The critical concentrations for the seven drugs used in the MGIT 960 system are as follows: amikacin, 1.0 microg/ml; capreomycin, 2.5 microg/ml; ethionamide, 5.0 microg/ml; protionamide, 2.5 microg/ml; ofloxacin, 2.0 microg/ml; rifabutin, 0.5 microg/ml; linezolid, 1.0 microg/ml. Our results demonstrate that the BACTEC MGIT 960 system is an accurate method for rapid testing of the susceptibilities of Mycobacterium tuberculosis to second-line drugs.

PubMed Disclaimer

References

    1. Balabanova, Y., M. Ruddy, J. Hubb, M. Yates, M. Malomanova, I. Fedorin, and F. Drobniewski. 2005. Multidrug-resistant tuberculosis in Russia: clinical characteristics, analysis of second-line drug resistance and development of standardized therapy. Eur. J. Clin. Microbiol. Infect. Dis. 24:136-139. - PubMed
    1. Bastian, I., L. Rigouts, J. C. Palomino, and F. Portaels. 2001. Kanamycin susceptibility testing of Mycobacterium tuberculosis using Mycobacterium Growth Indicator Tube and a colorimetric method. Antimicrob. Agents Chemother. 45:1934-1936. - PMC - PubMed
    1. Bemer, P., F. Palicova, S. Rüsch-Gerdes, H. B. Drugeon, and G. E. Pfyffer. 2002. Multicenter evaluation of fully automated BACTEC Mycobacteria Growth Indicator Tube 960 system for susceptibility testing of Mycobacterium tuberculosis. J. Clin. Microbiol. 40:150-154. - PMC - PubMed
    1. Goloubeva, V., M. Lecocq, P. Lassowsky, F. Matthys, F. Portaels, and I. Bastian. 2001. Evaluation of Mycobacteria Growth Indicator Tube for direct and indirect drug susceptibility testing of Mycobacterium tuberculosis from respiratory specimens in a Siberian prison hospital. J. Clin. Microbiol. 39:1501-1505. - PMC - PubMed
    1. Huang, T.-S., H.-Z. Tu, S. S.-J. Lee, W.-K. Huang, and Y.-C. Liu. 2002. Antimicrobial susceptibility testing of Mycobacterium tuberculosis to first-line drugs: comparisons of the MGIT 960 and BACTEC 460 systems. Ann. Clin. Lab. Sci. 32:142-147. - PubMed

MeSH terms

Substances

LinkOut - more resources