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. 2006 Mar;44(3):888-91.
doi: 10.1128/JCM.44.3.888-891.2006.

Reactivity of antibodies from syphilis patients to a protein array representing the Treponema pallidum proteome

Mary Beth Brinkman  1 , Matthew McKevitt, Melanie McLoughlin, Carla Perez, Jerrilyn Howell, George M Weinstock, Steven J Norris, Timothy Palzkill
Affiliations

Reactivity of antibodies from syphilis patients to a protein array representing the Treponema pallidum proteome

Mary Beth Brinkman et al. J Clin Microbiol. 2006 Mar.

Abstract

To identify antigens important in the human immune response to syphilis, the serum antibody reactivity of syphilitic patients was examined with 908 of the 1,039 proteins in the proteome of Treponema pallidum subsp. pallidum using a protein array enzyme-linked immunosorbent assay. Thirty-four proteins exhibited significant reactivity when assayed with human sera from patients in the early latent stage of syphilis. A subset of antigens identified were further scrutinized for antibody reactivity at primary, secondary, and latent disease stages, and the results demonstrate that the humoral immune response to individual T. pallidum proteins develops at different rates during the time course of infection.

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Figures

FIG. 1.
FIG. 1.
Identification of antigenic proteins in the T. pallidum proteome using sera collected from patients in the early latent stage of disease. The chemiluminescence ratio refers to the relative light units resulting from the binding of serum Ig to the T. pallidum-GST fusion protein divided by the value obtained when wells were coated with GST alone.
See this image and copyright information in PMC

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