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. 2006 Mar;44(3):1132-4.
doi: 10.1128/JCM.44.3.1132-1134.2006.

Detection of human bocavirus in Japanese children with lower respiratory tract infections

Xiaoming Ma  1 , Rika Endo, Nobuhisa Ishiguro, Takashi Ebihara, Hiroaki Ishiko, Tadashi Ariga, Hideaki Kikuta
Affiliations

Detection of human bocavirus in Japanese children with lower respiratory tract infections

Xiaoming Ma et al. J Clin Microbiol. 2006 Mar.

Abstract

Human bocavirus (HBoV), a newly cloned human virus of the genus Bocavirus, was detected by PCR from nasopharyngeal swab samples (8 of 318; 5.7%) collected from children with lower respiratory tract infections. HBoV may be one of the causative agents of lower respiratory tract infections in young children.

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Figures

FIG. 1.
FIG. 1.
Sequences of PCR products of HBoV-positive samples. A of the ATG initiator methionine codon of NP-1 protein is denoted as nucleotide +1. The sequences of the 18 samples were divided into three groups and compared with that of the published sequence of HBoV (GenBank accession number DQ000495) as a reference. Group 1 (JPBS03-112, JPBS03-217, JPBS03-219, JPBS03-261, JPBS03-298, JPBS05-7, JPBS05-18, JPBS05-28, JPBS05-29, JPBT03-5, JPBT03-12, JPBT03-43, JPBT05-46, and JPBT05-52) was completely identical to the reference. Group 2 (JPBS03-182, JPBS03-207, and JPBS03-208) had a single-base-pair substitution (176G to A) in the NP-1 gene, resulting in an amino acid exchange (Ser to Asn). Group 3 (JPBS03-98) had a single-base-pair substitution (201G to A) in the NP-1 gene without an amino acid exchange.
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