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. 1991 Jun 1;65(1):31-5.
doi: 10.1016/0378-1097(91)90466-n.

Sequence of an operon containing a novel delta-endotoxin gene from Bacillus thuringiensis

D Wu  1 , X L Cao, Y Y Bai, A I Aronson
Affiliations

Sequence of an operon containing a novel delta-endotoxin gene from Bacillus thuringiensis

D Wu et al. FEMS Microbiol Lett. .

Abstract

An insect larval toxin designated CryII is produced by several subspecies of Bacillus thuringiensis and differs from the other major delta-endotoxins in these bacteria in its size, toxicity profile and presence as part of an operon with three open reading frames (ORF). Such an operon from a novel B. thuringiensis isolate has been cloned and differs from one previously characterized in the following ways: (a) the size and number of amino acid repeats in one of the ORFs; (b) the smaller size of the CryII protoxin and the presence of a unique 110-kDa CryII-related antigen; and (c) high larvicidal activity for a particular Lepidopteran but low activity for a Dipteran. Various subclones of this operon were introduced into a plasmid-free B. thuringiensis strain and only the cryII gene was found to be necessary for protoxin accumulation.

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