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. 2006 Mar 10:7:14.
doi: 10.1186/1471-2121-7-14.

Aging of mesenchymal stem cell in vitro

Mandana Mohyeddin Bonab  1 , Kamran Alimoghaddam, Fatemeh Talebian, Syed Hamid Ghaffari, Ardeshir Ghavamzadeh, Behrouz Nikbin
Affiliations

Aging of mesenchymal stem cell in vitro

Mandana Mohyeddin Bonab et al. BMC Cell Biol. .

Abstract

Background: A hot new topic in medical treatment is the use of mesenchymal stem cells (MSC) in therapy. The low frequency of this subpopulation of stem cells in bone marrow (BM) necessitates their in vitro expansion prior to clinical use. We evaluated the effect of long term culture on the senescence of these cells.

Results: The mean long term culture was 118 days and the mean passage number was 9. The average number of PD decreased from 7.7 to 1.2 in the 10th passage. The mean telomere length decreased from 9.19 Kbp to 8.7 kbp in the 9th passage. Differentiation potential dropped from the 6th passage on. The culture's morphological abnormalities were typical of the Hayflick model of cellular aging.

Conclusion: We believe that MSC enter senescence almost undetectably from the moment of in vitro culturing. Simultaneously these cells are losing their stem cell characteristics. Therefore, it is much better to consider them for cell and gene therapy early on.

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Figures

Figure 1
Figure 1
a) Bone Marrow MSC in conventional culture (adherent, elongated cells); b) Oil red O staining showing fat accumulation in adipocytes. c & d) Van Kossas and Alkaline Phosphatase staining showing osteocytes differentiation of MSC.
Figure 2
Figure 2
Long term growth curves; each obtained from an individual donor.
Figure 3
Figure 3
Southern-blot analysis of telomere lengths of expanded MSCs derived from two BM samples during multiple passages (CH = Control-DNA, High, CL = Control-DNA, Low, P = Passage).
See this image and copyright information in PMC

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