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. 1998 Mar 1;70(5):179A-85A.
doi: 10.1021/ac981773x.

Tandem FTMS of Large Biomolecules

E R Williams  1
Affiliations

Tandem FTMS of Large Biomolecules

E R Williams. Anal Chem. .
No abstract available

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Figures

Figure 1
Figure 1. Schematic of an external ion-source FTMS instrument
Electrospray ions generated at atmospheric pressure are guided through five differential pumping stages and into the ion cell at 10−9 Torr by means of an electrostatic ion guide system. Neutral atoms or molecules are introduced through the leak or pulsed valves for collisional activated dissociation or ion-molecule reactions; photodissociation occurs with lasers or blackbody photons from the heated vacuum chamber.
Figure 2
Figure 2. ESI-FTMS spectrum of chondroitinase II
(a) Entire charge-state distribution produced by electrospray, (b) (M + 90H)90+ ions detected over a narrow bandwidth for high resolution, and (c) data from (b) after time-domain sampling, showing isotopically resolved molecular ions, adducts, and fragmentation products. (Adapted with permission from Ref. .)
Figure 3
Figure 3. ESI-FTMS spectrum of bovine carbonic anhydrase II bound with inhibitors
(a) Entire charge-state distribution produced by electrospray, (b) after ion isolation and collisional dissociation, and (c) isolation and dissociation of three inhibitors. (Adapted from Ref. .)
Figure 4
Figure 4. Partial IRMPD spectrum of a DNA 50-mer
Insets show isotopic distributions for selected ions; ions were irradiated for 10 ms using a CO2 laser with a 10.6-μm wavelength. (Adapted from Ref. .)
Figure 5
Figure 5. Blackbody IR radiative dissociation spectra of ubiquitin
(a) (M + 11H)11+ and (b)(M + 5H)5+ ions taken from a series of measurements at delay tirnes. (Adapted from Ref. .)
None
See this image and copyright information in PMC

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