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. 1991 Sep;173(17):5551-3.
doi: 10.1128/jb.173.17.5551-5553.1991.

Gyrase inhibitors increase the content of knotted DNA species of plasmid pBR322 in Escherichia coli

S Ishii  1 , T Murakami, K Shishido
Affiliations

Gyrase inhibitors increase the content of knotted DNA species of plasmid pBR322 in Escherichia coli

S Ishii et al. J Bacteriol. 1991 Sep.

Abstract

Treatment of Escherichia coli cells harboring pBR322 with the DNA gyrase inhibitors oxolinic acid and coumermycin A1 led to an increase in the content of knotted pBR322 molecules. This phenomenon was attributed to inhibition of gyrase-catalyzed unknotting of the plasmid DNA knotted by transcription.

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