Cytogenetic mapping of 11 functional genes to chicken chromosomes by fluorescence in situ hybridization

Abstract

With chicken bacterial artificial chromosome (BAC) DNA as probes, 11 non-assigned functional genes were localized to chicken chromosomes 1 or 2 by fluorescence in situ hybridization (FISH). The 11 genes and their chromosomal positions are as follows: ALVEB5, 1p26-24; ACO2, 1p16-14; HSP108, 1p14-13; CD4, 1q11; HSD3B; 1q11; SOD1, 1q14-21; LAMP1, 1q24-31; P2Y5, 1q35-36; EN2, 2p31-24; NPY, 2p14-13 and CA2, 2q31-32. Metaphase chromosome spreads used for hybridization were prepared from embryonic chicken fibroblast cultures. The gene position was identified according to the international standardized G-banded karyotype of chicken by measuring the relative fractional length from the telomere of the p-arm to the hybridization signal (FLpter). The 11 genes mapped newly will enrich the cytogenetic map and serve as additional anchor markers for integrating the cytogenetic map with the genetic map of chicken.