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. 1995 Aug;61(8):3189-91.
doi: 10.1128/aem.61.8.3189-3191.1995.

Selection and Characterization of Aspartokinase Feedback-Insensitive Mutants of Azotobacter vinelandii

C R Ekechukwu, T A Burns, T Melton

Selection and Characterization of Aspartokinase Feedback-Insensitive Mutants of Azotobacter vinelandii

C R Ekechukwu et al. Appl Environ Microbiol. 1995 Aug.

Abstract

Aspartokinase feedback-insensitive mutants of Azotobacter vinelandii were selected as resistant to l-threonine, (beta)-hydroxynorvaline, or S-(2-aminoethyl)-l-cysteine. l-Threonine-resistant strains were classified into three groups based on their ability to transport l-threonine and their growth response to O-methylthreonine and (beta)-hydroxynorvaline. Most of the mutants were transport defective; however, some were desensitized to feedback regulation.

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