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. 1995 Sep;61(9):3256-60.
doi: 10.1128/aem.61.9.3256-3260.1995.

Use of Electroporation To Generate a Thiobacillus neapolitanus Carboxysome Mutant

R S English, S Jin, J M Shively

Use of Electroporation To Generate a Thiobacillus neapolitanus Carboxysome Mutant

R S English et al. Appl Environ Microbiol. 1995 Sep.

Abstract

Two cloning vectors designed for use in Escherichia coli and the thiobacilli were constructed by combining a Thiobacillus intermedius plasmid replicon with a multicloning site, lacZ(prm1), and either a kanamycin or a streptomycin resistance gene. Conditions necessary for the introduction of DNA into T. intermedius and T. neapolitanus via electroporation were examined and optimized. By using optimal electroporation conditions, the gene encoding a carboxysome shell protein, csoS1A, was insertionally inactivated in T. neapolitanus. The mutant showed a reduced number of carboxysomes and an increased level of CO(inf2) necessary for growth.

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