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. 1996 Aug;62(8):3050-2.
doi: 10.1128/aem.62.8.3050-3052.1996.

Quantification of Dehalospirillum multivorans in Mixed-Culture Biofilms with an Enzyme-Linked Immunosorbent Assay

P Bauer-Kreisel, M Eisenbeis, H Scholz-Muramatsu

Quantification of Dehalospirillum multivorans in Mixed-Culture Biofilms with an Enzyme-Linked Immunosorbent Assay

P Bauer-Kreisel et al. Appl Environ Microbiol. 1996 Aug.

Abstract

A fast, highly selective and sensitive method to quantify specific biomasses in mixed-culture biofilms is described. It consists of detachment of a biofilm from its support material, resolution of the detached biofilm flocs in order to separate the enclosed cells and antigens, and quantification of specific biomass by an enzyme-linked immunosorbent assay.

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