Purification and Characterization of a Proteasome from the Hyperthermophilic Archaeon Pyrococcus furiosus
- PMID: 16535545
- PMCID: PMC1389139
- DOI: 10.1128/aem.63.3.1160-1164.1997
Purification and Characterization of a Proteasome from the Hyperthermophilic Archaeon Pyrococcus furiosus
Abstract
A 640-kDa proteasome consisting of (alpha) (25-kDa) and (beta) (22-kDa) subunits, and with a temperature optimum of 95(deg)C, was purified from crude cell extracts of a hyperthermophilic archaeon, Pyrococcus furiosus. Although this is the fourth member of the kingdom Euryarchaeota (and the first hyperthermophile) found to contain a proteasome, none has been identified among the members of the kingdom Crenarchaeota.
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