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. 1997 Mar;63(3):862-6.
doi: 10.1128/aem.63.3.862-866.1997.

Dechlorination of Atrazine by a Rhizobium sp. Isolate

C Bouquard, J Ouazzani, J Prome, Y Michel-Briand, P Plesiat

Dechlorination of Atrazine by a Rhizobium sp. Isolate

C Bouquard et al. Appl Environ Microbiol. 1997 Mar.

Abstract

A Rhizobium sp. strain, named PATR, was isolated from an agricultural soil and found to actively degrade the herbicide atrazine. Incubation of PATR in a basal liquid medium containing 30 mg of atrazine liter(sup-1) resulted in the rapid consumption of the herbicide and the accumulation of hydroxyatrazine as the only metabolite detected after 8 days of culture. Experiments performed with ring-labeled [(sup14)C]atrazine indicated no mineralization. The enzyme responsible for the hydroxylation of atrazine was partially purified and found to consist of four 50-kDa subunits. Its synthesis in PATR was constitutive. This new atrazine hydrolase demonstrated 92% sequence identity through a 24-amino-acid fragment with atrazine chlorohydrolase AtzA produced by Pseudomonas sp. strain ADP.

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