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. 2006 Sep;65(9):1219-22.
doi: 10.1136/ard.2005.049403. Epub 2006 Mar 15.

Citrullination is an inflammation-dependent process

D Makrygiannakis  1 , E af Klint, I E Lundberg, R Löfberg, A-K Ulfgren, L Klareskog, A I Catrina
Affiliations

Citrullination is an inflammation-dependent process

D Makrygiannakis et al. Ann Rheum Dis. 2006 Sep.

Abstract

Objectives: To study the presence of citrullinated proteins in inflammatory conditions and in clinically non-affected tissues of controls.

Methods: Synovial biopsy specimens from 19 patients with rheumatoid arthritis and 10 healthy controls were investigated by immunohistochemistry. Additionally, muscle tissue from 5 patients with polymyositis and from 7 healthy controls, intestinal tissue from macroscopically affected and non-affected areas from 10 patients with inflammatory bowel disease (IBD) and tonsil tissues from 4 chronically inflamed tonsils were studied.

Results: Citrullinated proteins were present in all synovial biopsy specimens from patients with rheumatoid arthritis, whereas only three of 10 healthy synovial biopsy specimens showed scarce amounts of citrullination. Citrullination was also present in all myositis-affected muscles, whereas it was absent in the muscle tissues of controls. All tonsil biopsy specimens studied were positive for citrulline. Even though more frequently detected in the macroscopically affected colonic areas, no marked difference was observed in the pattern or extent of citrullination between the macroscopically affected and non-affected intestinal IBD tissues.

Conclusion: Citrullination is present in a wide range of inflammatory tissues, suggesting that this process is inflammation dependent rather than disease dependent.

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Conflict of interest statement

Competing interests: None declared.

References

    1. Klareskog L, Stolt P, Lundberg K, Kallberg H, Bengtsson C, Grunewald J.et al A new model for an etiology of rheumatoid arthritis: smoking may trigger HLA‐DR (shared epitope)‐restricted immune reactions to autoantigens modified by citrullination. Arthritis Rheum 20065438–46. - PubMed
    1. Tsuji Y, Akiyama M, Arita K, Senshu T, Shimizu H. Changing pattern of deiminated proteins in developing human epidermis. J Invest Dermatol 2003120817–822. - PubMed
    1. Tarcsa E, Marekov L N, Andreoli J, Idler W W, Candi E, Chung S I.et al The fate of trichohyalin. Sequential post‐translational modifications by peptidyl‐arginine deiminase and transglutaminases. J Biol Chem 199727227893–27901. - PubMed
    1. Moscarello M A, Wood D D, Ackerley C, Boulias C. Myelin in multiple sclerosis is developmentally immature. J Clin Invest 199494146–154. - PMC - PubMed
    1. Baeten D, Peene I, Union A, Meheus L, Sebbag M, Serre G.et al Specific presence of intracellular citrullinated proteins in rheumatoid arthritis synovium: relevance to antifilaggrin autoantibodies. Arthritis Rheum 2001442255–2262. - PubMed

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