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Review
. 2006 Jun;23(2-3):89-110.
doi: 10.1016/j.bioeng.2006.02.003. Epub 2006 Mar 20.

Protein engineering of formate dehydrogenase

Vladimir I Tishkov  1 , Vladimir O Popov
Affiliations
Review

Protein engineering of formate dehydrogenase

Vladimir I Tishkov et al. Biomol Eng. 2006 Jun.

Abstract

NAD+-dependent formate dehydrogenase (FDH, EC 1.2.1.2) is one of the best enzymes for the purpose of NADH regeneration in dehydrogenase-based synthesis of optically active compounds. Low operational stability and high production cost of native FDHs limit their application in commercial production of chiral compounds. The review summarizes the results on engineering of bacterial and yeast FDHs aimed at improving their chemical and thermal stability, catalytic activity, switch in coenzyme specificity from NAD+ to NADP+ and overexpression in Escherichia coli cells.

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