Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Mar;2(3):319-25.
doi: 10.1093/nar/2.3.319.

Determination of the 3' terminal nucleotide of DNA fragments

K Olson, C Harvey
Free PMC article

Determination of the 3' terminal nucleotide of DNA fragments

K Olson et al. Nucleic Acids Res. 1975 Mar.
Free PMC article

Abstract

A new method for determining the 3'-terminal nucleotide of a DNA strand is presented. Use is made of the fact that one (and only one) 2',3'-dideoxyribonucleotide can be added to the 3'-end of a DNA fragment with calf thymus terminal transferase. Addition of more than one nucleotide analog per strand is impossible due to the absence of a 3'-terminal hydroxyl group. If the terminatind dideoxyribonucleotide contains an (alpha 32p) label, the resulting 3'-blocked strand can be digested by "nearest neighbor" techniques and the original 3'-endgroup determined. Picomole quantities of DNA strands can be labeled and the 3'-end determined.

PubMed Disclaimer

References

    1. J Biol Chem. 1964 Nov;239:3885-92 - PubMed
    1. Biochemistry. 1969 Dec;8(12):4897-904 - PubMed
    1. Eur J Biochem. 1971 Sep 24;22(2):271-6 - PubMed
    1. J Am Chem Soc. 1973 Jun 13;95(12):4025-30 - PubMed
    1. Biochemistry. 1973 Jan 16;12(2):208-14 - PubMed