Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Mar;72(3):1189-93.
doi: 10.1073/pnas.72.3.1189.

Methylation-dependent translation of viral messenger RNAs in vitro

G W Both, A K Banerjee, A J Shatkin

Methylation-dependent translation of viral messenger RNAs in vitro

G W Both et al. Proc Natl Acad Sci U S A. 1975 Mar.

Abstract

Methylated reovirus and vesicular stomatitis virus mRNAs, synthesized in vitro in the presence of S-adenosylmethionine by the virion-associated polymerases (RNA nucleotidyltransferases, EC 2.7.7.6), stimulate protein synthesis by wehat germ extracts to a greater extent than unmethylated mRNAs. Addition of S-adenosylmethionine to a cell-free extract programmed with unmethylated mRNA stimulates protein synthesis and results in methylation of the mRNA. An inhibitor of mRNA methylation. S-adenosylhomocysteine, blocks translation of unmethylated, but not of methylated, mRNAs. Aurintricarboxylic acid, which inhibits polypepetide chain initiation, also prevents mRNA methylation by wheat germ extracts. In contrast, sparsomycin, which inhibits polypeptide chain elongation, does not reduce mRNA methylation. The results indicate that methylation of viral mRNA is required for translation in vitro and suggest that mRNA methylation occurs at the initiation step of protein synthesis.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1975 Jan;72(1):362-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Feb;72(2):742-5 - PubMed
    1. Nucleic Acids Res. 1974 Jun;1(6):809-22 - PubMed
    1. Cell. 1975 Feb;4(2):173-80 - PubMed
    1. FEBS Lett. 1975 Jan 1;49(3):385-9 - PubMed

MeSH terms