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. 2006 Apr;100(4):641-7.
doi: 10.1111/j.1365-2672.2005.02791.x.

Thermophilic protease production by Streptomyces sp. 594 in submerged and solid-state fermentations using feather meal

L A I De Azeredo  1 , M B De Lima, R R R Coelho, D M G Freire
Affiliations

Thermophilic protease production by Streptomyces sp. 594 in submerged and solid-state fermentations using feather meal

L A I De Azeredo et al. J Appl Microbiol. 2006 Apr.

Abstract

Aims: Protease production by Streptomyces sp. 594 in submerged (SF) and solid-state fermentation (SSF) using feather meal, an industrial poultry residue, and partial characterization of the crude enzyme.

Methods and results: Streptomyces sp. 594 produced proteases in SF (7.2 +/- 0.2 U ml(-1)) and SSF (15.5 +/- 0.41 U g(-1)), with pH increase in both media. Considering protease activity, values obtained in the liquid extract after SSF (6.3 +/- 0.17 U ml(-1)) were lower than those from SF. The proteases, which belong to serine and metalloproteinase classes, were active over a wide range of pH (5.0-10.0) and high temperatures (55-80 degrees C). Strain 594 was also able to degrade feather in agar and liquid media. Keratinase activity (80 U l(-1)) also confirmed the keratin degrading capacity of this streptomycete.

Conclusions: Proteases produced using residues from poultry industry have shown interesting properties for industrial purposes.

Significance and impact of the study: As far as we are concerned, this is the first contribution towards the production of thermophilic protease by a streptomycete in SSF using a keratinous waste.

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