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. 2006 Apr;3(4):259-61.
doi: 10.1038/nmeth862.

Imaging specific cell-surface proteolytic activity in single living cells

John P Hobson  1 , Shihui Liu, Birgitte Rønø, Stephen H Leppla, Thomas H Bugge
Affiliations

Imaging specific cell-surface proteolytic activity in single living cells

John P Hobson et al. Nat Methods. 2006 Apr.

Abstract

We describe a simple, sensitive and noninvasive assay that uses nontoxic, reengineered anthrax toxin-beta-lactamase fusion proteins with altered protease cleavage specificity to visualize specific cell-surface proteolytic activity in single living cells. The assay could be used to specifically image endogenous cell-surface furin, urokinase plasminogen activator and metalloprotease activity. We have adapted the assay for fluorescence microscopy, flow cytometry and fluorescent plate reader formats, and it is amenable for automation and high-throughput analysis.

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