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. 1973 Mar;7(3):356-60.
doi: 10.1128/iai.7.3.356-360.1973.

A simplified method for immunological typing of trachoma-inclusion conjunctivitis-lymphogranuloma venereum organisms

S P Wang  1 , C C Kuo, J T Grayston
Affiliations

A simplified method for immunological typing of trachoma-inclusion conjunctivitis-lymphogranuloma venereum organisms

S P Wang et al. Infect Immun. 1973 Mar.

Abstract

Responding to the pressure of large numbers of trachoma-inclusion conjunctivitis (TRIC)-lymphogranuloma venereum (LGV) isolates from field studies requiring serotyping, we have developed a simplified, less-precise method that utilizes cell culture-grown organisms to produce mouse antisera which is tested agaist prototype TRIC-LGV antigens in the micro-immunofluorescence test. Cell cultures with as few as 5 to 15% of cells showing inclusions produced adequate antibody in mice 4 days after single injection. Knowledge of the reaction of prototype antisera with the antigens has allowed typing of most isolates tested from the pattern of cross-reaction of their antiserum.

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References

    1. J Infect Dis. 1969 Oct;120(4):451-62 - PubMed
    1. J Gen Microbiol. 1960 Dec;23:613-9 - PubMed
    1. Proc Soc Exp Biol Med. 1964 Mar;115:587-91 - PubMed
    1. J Infect Dis. 1972 Jun;125(6):665-8 - PubMed
    1. Am J Ophthalmol. 1970 Sep;70(3):367-74 - PubMed

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