doi: 10.1007/BF00227751.
Brown adipose tissue mitochondria are cytochrome c-subsaturated
Affiliations
- PMID: 1656204
- DOI: 10.1007/BF00227751
Item in Clipboard
Brown adipose tissue mitochondria are cytochrome c-subsaturated
Mol Cell Biochem.
.
Abstract
The oxidative activity of mitochondria freshly isolated from brown adipose tissue of rats was stimulated two-fold on the addition of small concentrations of exogenous cytochrome c to the reaction medium. Loss of membrane-bound cytochrome c did not occur during isolation of mitochondria. Estimation of the high-affinity binding sites on the organelle membrane indicated that less than a third of these sites remained saturated with cytochrome c. The pigment is thus shown to be a functionally limiting electron transport component in brown adipose tissue.
Similar articles
-
Regulation of respiratory activity of brown adipose tissue mitochondria through changes in cytochromes.Biotechnol Appl Biochem. 1990 Oct;12(5):562-6. Biotechnol Appl Biochem. 1990. PMID: 2288713
-
Isolation and subfractionation of mitochondria from animal cells and tissue culture lines.Methods Cell Biol. 2001;65:1-35. doi: 10.1016/s0091-679x(01)65002-7. Methods Cell Biol. 2001. PMID: 11381588 No abstract available.
-
Effects of training and exhaustive exercise on the mitochondrial oxidative capacity of brown adipose tissue.Biosci Rep. 1984 Nov;4(11):987-93. doi: 10.1007/BF01116898. Biosci Rep. 1984. PMID: 6098322
-
Functions of cytochrome c in regulation of electron transfer and protein folding.Indian J Biochem Biophys. 1992 Apr;29(2):173-8. Indian J Biochem Biophys. 1992. PMID: 1328035 Review.
-
Brown adipose tissue mitochondria.Biochim Biophys Acta. 1975 Mar 31;416(1):53-103. doi: 10.1016/0304-4173(75)90013-0. Biochim Biophys Acta. 1975. PMID: 235330 Review. No abstract available.
Cited by
-
The pro-apoptotic proteins, Bid and Bax, cause a limited permeabilization of the mitochondrial outer membrane that is enhanced by cytosol.J Cell Biol. 1999 Nov 15;147(4):809-22. doi: 10.1083/jcb.147.4.809. J Cell Biol. 1999. PMID: 10562282 Free PMC article.
-
Modeling kinetics of subcellular disposition of chemicals.Chem Rev. 2009 May;109(5):1793-899. doi: 10.1021/cr030440j. Chem Rev. 2009. PMID: 19265398 Free PMC article. Review. No abstract available.