Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Nov;11(11):5586-91.
doi: 10.1128/mcb.11.11.5586-5591.1991.

The length of homology required for gene targeting in embryonic stem cells

P Hasty  1 , J Rivera-Pérez, A Bradley
Affiliations

The length of homology required for gene targeting in embryonic stem cells

P Hasty et al. Mol Cell Biol. 1991 Nov.

Abstract

Homologous recombination has been used to introduce site-specific mutations into murine embryonic stem (ES) cells with both insertion and replacement vectors. In this study, we compared the frequency of gene targeting with various lengths of homology and found a dramatic increase in targeting with an increase in homology from 1.3 to 6.8 kb. We examined in detail the relationship between the length of homology and the gene-targeting frequency for replacement vectors and found that a critical length of homology is needed for targeting. Adding greater lengths of homology to this critical length has less of an effect on the targeting frequency. We also analyzed the lengths of homology necessary on both arms of the vector for gene replacement events and found that 472 bp of homology is used as efficiently as 1.2 kb in the formation and resolution of crossover junctions.

PubMed Disclaimer

References

    1. Nature. 1991 Mar 21;350(6315):243-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6354-8 - PubMed
    1. Cell. 1991 Feb 22;64(4):693-702 - PubMed
    1. Science. 1989 Sep 15;245(4923):1234-6 - PubMed
    1. Cell. 1987 Nov 6;51(3):503-12 - PubMed

Publication types