Microcystin production in benthic mats of cyanobacteria in the Nile River and irrigation canals, Egypt

Abstract

The present study describes for the first time the species composition and toxicity of benthic cyanobacteria forming mats on the Nile River and irrigation canal sediments in Egypt. A total of 19 species of cyanobacteria were isolated from these mats during this study. The toxicity of the extracts of these species was investigated using Artemia salina assay, mouse bioassay and enzyme linked immunosorbent assay (ELISA). The results showed that all the 19 benthic species isolated from cyanobacterial mats, were toxic to A. salina. Two of these species, namely Calothrix parietina and Phormidium tenue, caused toxicity to mice with neurotoxic signs appeared within 12 h after injection. Whereas, five species showed hepatotoxic effects to mice within 6 h after injection. The results of ELISA showed that all the extracts which had hepatotoxic effects to mice, contained high levels of microcystins with concentrations ranging from 1.6 to 4.1 mg g(-1) dry weight. HPLC analysis for heptotoxic extracts revealed that these extracts contained two peaks corresponding to microcystin-YR and -LR with different proportions. This study suggests that benthic species should be considered along with planktonic species during monitoring of toxic cyanobacteria in water sources, particularly the Nile river which is the main source of drinking water in Egypt.