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. 1991 Jun;20(1):45-58.
doi: 10.1016/0168-1702(91)90060-9.

Neurovirulence of six different murine coronavirus JHMV variants for rats

Y Matsubara  1 , R Watanabe, F Taguchi
Affiliations

Neurovirulence of six different murine coronavirus JHMV variants for rats

Y Matsubara et al. Virus Res. 1991 Jun.

Abstract

Six variant viruses of the JHMV strain of murine coronavirus with large (cl-2, CNSV, DL and DS) or small (sp-4 and JHM-X) S proteins were compared in terms of their relative neurovirulence in weanling Lewis rats. Inoculation of various doses of the variants revealed that the cl-2 and CNSV were highly virulent and DL and DS were low-virulent, while sp-4 and JHM-X were avirulent. Pathological examination of rats infected with variants cl-2, DL and sp-4 showed that the cl-2 and DL induced severe and mild acute encephalomyelitis, respectively, while no lesions were observed in the central nervous system of rats infected with sp-4. Virus growth and distribution of antigen in rat brains correlated strongly with neurovirulence. These results suggest that S protein plays a role in neurovirulence in rats. In addition, these variant viruses were shown to be useful tools for further analysis of JHMV neurovirulence in animals as well as in cultured cells.

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