JC virus detection by in situ hybridization in brain tissue from elderly patients
- PMID: 1656842
- DOI: 10.1002/ana.410290414
JC virus detection by in situ hybridization in brain tissue from elderly patients
Abstract
Brains from 10 patients aged 68 to 96 years at time of death were studied for JC viral DNA and common papovaviral capsid protein. In situ hybridization of JC viral DNA was performed by affinity cytochemistry using a biotinylated fragment of JC viral DNA. Immunohistochemistry was performed on brain tissue by the avidin DH-biotinylated horseradish peroxidase technique using polyclonal antibody raised against the papovaviral capsid protein. Viral protein and DNA were detected in 4 of 10 patients. JC virus may be present in the brains of aged patients more frequently than previously suspected.
Comment in
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Is JC virus latent in brain?Ann Neurol. 1991 Apr;29(4):433-4. doi: 10.1002/ana.410290415. Ann Neurol. 1991. PMID: 1656843 No abstract available.
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JC virus DNA in healthy brain tissue: a challenge for progressive multifocal leukoencephalopathy diagnosis.Ann Neurol. 2009 Feb;65(2):230; author reply 230-31. doi: 10.1002/ana.21547. Ann Neurol. 2009. PMID: 19259969 No abstract available.
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