Comparative Study
doi: 10.1016/s0006-291x(05)81251-3.
cDNA cloning of the beta-subunit of the human gastric H,K-ATPase
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- PMID: 1656976
- DOI: 10.1016/s0006-291x(05)81251-3
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Comparative Study
cDNA cloning of the beta-subunit of the human gastric H,K-ATPase
Biochem Biophys Res Commun.
.
Abstract
A full-length cDNA clone encoding the human gastric H,K-ATPase (EC 3.6.1.36)beta-subunit was isolated from a human gastric mucosal lambda gt10 library using oligonucleotide probes which were based on the cDNA sequence from rat and rabbit H,K-ATPase beta-subunits. The insert was 1407 bp in length and encoded a polypeptide of 291 amino acids with a MW = 33,367 Da. It exhibited 84.2%, 85.6% and 81.3% identity to the H,K-ATPase beta-subunits of rabbit, pig and rat, respectively.
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