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. 2006 Mar 29:4:4.
doi: 10.1186/1477-5956-4-4.

Proteins pattern alteration in AZT-treated K562 cells detected by two-dimensional gel electrophoresis and peptide mass fingerprinting

Gabriele D'Andrea  1 , Anna R Lizzi, Sara Venditti, Laura Di Francesco, Alessandra Giorgi, Giuseppina Mignogna, Arduino Oratore, Argante Bozzi
Affiliations

Proteins pattern alteration in AZT-treated K562 cells detected by two-dimensional gel electrophoresis and peptide mass fingerprinting

Gabriele D'Andrea et al. Proteome Sci. .

Abstract

In this study we report the effect of AZT on the whole protein expression profile both in the control and the AZT-treated K562 cells, evidenced by two-dimensional gel electrophoresis and peptide mass fingerprinting analysis. Two-dimensional gels computer digital image analysis showed two spots that appeared up-regulated in AZT-treated cells and one spot present only in the drug exposed samples. Upon extraction and analysis by peptide mass fingerprinting, the first two spots were identified as PDI-A3 and stathmin, while the third one was proved to be NDPK-A. Conversely, two protein spots were present only in the untreated K562 cells, and were identified as SOD1 and HSP-60, respectively.

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Figures

Figure 1
Figure 1
Representative Coomassie Blue G250-stained 2DE gel of K562 cells, developed in the IPG 3–10 linear interval. Left side: control K562 cells; right side: AZT-treated K562 cells. 1: HSP-60; 2: PDI-A3; 3: NDPK-A; 4: Stathmin; 5: SOD1. n.i.: not identified.
See this image and copyright information in PMC

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