From yeast ammonium transporters to Rhesus proteins, isolation and functional characterization
- PMID: 16574457
- DOI: 10.1016/j.tracli.2006.03.002
From yeast ammonium transporters to Rhesus proteins, isolation and functional characterization
Abstract
Saccharomyces cerevisiae possesses three ammonium transporters from the Mep/Amt family involved in ammonium acquisition and retention. We have shown that Rh proteins are structurally related to Mep/Amt proteins and that human RhAG and RhCG perform bi-directional ammonium transport upon heterologous expression in yeast. Using yeast as an expression tool, we have started a structure-function analysis of distinct members from the Mep/Amt/Rh super-family.
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