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. 1991 Sep;82(9):965-8.
doi: 10.1111/j.1349-7006.1991.tb01928.x.

Ki-ras activation in pancreatic carcinomas of Syrian hamsters induced by N-nitrosobis(2-hydroxypropyl)amine

T Ushijima  1 , M Tsutsumi, R Sakai, Y Ishizaka, F Takaku, Y Konishi, M Takahashi, T Sugimura, M Nagao
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Ki-ras activation in pancreatic carcinomas of Syrian hamsters induced by N-nitrosobis(2-hydroxypropyl)amine

T Ushijima et al. Jpn J Cancer Res. 1991 Sep.

Abstract

Five pancreatic carcinomas induced by N-nitrosobis(2-hydroxypropyl)amine in Syrian golden hamsters were analyzed for activation of Ki-ras at codons 12 and 13, using the polymerase chain reaction and direct sequencing. The Ki-ras gene was shown to be activated in four of the five carcinomas, and the results were further confirmed by subcloning and sequencing. All the mutations involved a G-to-A transition at the second position of codon 12, which resulted in a change at the amino acid level from glycine to aspartic acid. This mutation is identical with that reported for pancreatic tumors of Syrian hamsters induced by N-nitrosobis(2-oxopropyl)amine.

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