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. 1991 Nov;173(21):7029-32.
doi: 10.1128/jb.173.21.7029-7032.1991.

Purification and partial characterization of 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase and 7,8-dihydropteroate synthase from Escherichia coli MC4100

T L Talarico  1 , I K Dev, W S Dallas, R Ferone, P H Ray
Affiliations

Purification and partial characterization of 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase and 7,8-dihydropteroate synthase from Escherichia coli MC4100

T L Talarico et al. J Bacteriol. 1991 Nov.

Abstract

The enzymes 7,8-dihydroxymethylpterin-pyrophosphokinase (HPPK) and 7,8-dihydropteroate synthase (DHPS), which act sequentially in the folate pathway, were purified to homogeneity from crude extracts of Escherichia coli MC4100. The enzymes represent less than 0.01% of the total soluble protein. HPPK was purified greater than 10,000-fold; the native enzyme appears to be a monomer with a molecular mass of 25 kDa and a pI of 5.2. DHPS was purified greater than 7,000-fold; the native enzyme has an apparent molecular mass of 52 to 54 kDa and is composed of two identical 30-kDa subunits. The amino-terminal sequences for both enzymes have been determined.

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References

    1. J Biol Chem. 1956 Jan;218(1):97-106 - PubMed
    1. Anal Biochem. 1976 May 7;72:248-54 - PubMed
    1. J Biol Chem. 1964 Jul;239:2259-66 - PubMed
    1. J Biol Chem. 1964 Jan;239:326-31 - PubMed
    1. Biochim Biophys Acta. 1961 Sep 30;52:467-73 - PubMed

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