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. 1983 Feb;80(3):683-6.
doi: 10.1073/pnas.80.3.683.

Procedure for rapid isolation of photosynthetic reaction centers using cytochrome c affinity chromatography

G W Brudvig  1 , S T Worland, K Sauer
Affiliations

Procedure for rapid isolation of photosynthetic reaction centers using cytochrome c affinity chromatography

G W Brudvig et al. Proc Natl Acad Sci U S A. 1983 Feb.

Abstract

Horse heart cytochrome c linked to Sepharose 4B is used to purify reaction centers from Rhodopseudomonas sphaeroides R-26. This procedure allows for an initial recovery of 80-90% of the bacterial reaction centers present in chromatophore membranes. High purity reaction centers (A(280)/A(802) < 1.30) can be obtained with a 30% recovery. Reaction centers from wild-type Rps. sphaeroides and Rps. capsulata also bind to a cytochrome c column. Cytochrome c affinity chromatography can also be used to isolate photosystem I complexes from spinach chloroplasts.

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