Evaluation of antimicrobial susceptibility patterns in Campylobacter spp isolated from dairy cattle and farms managed organically and conventionally in the midwestern and northeastern United States

Abstract

Objective: To describe antimicrobial susceptibility patterns in Campylobacter spp isolated from dairy cattle and farms managed organically and conventionally in the midwestern and northeastern United States.

Design: Longitudinal study.

Sample population: 128 farms.

Procedure: Samples and data were collected every 2 months from August 2000 to October 2001. Fecal samples were collected from calves and cows. Milk samples were obtained from the bulk tank and milk line filters. Environmental samples were obtained from a water source, feed bunks of lactating cows, and cattle housing areas. Campylobacter identification and antimicrobial susceptibility testing were performed at a central laboratory by use of microbroth dilution with 2 customized antimicrobial susceptibility panels.

Results: 460 and 1,570 Campylobacter isolates were obtained from organic and conventional dairy farms, respectively. Most isolates from both farm types were susceptible to most antimicrobial agents tested, and antimicrobial susceptibility of conventional dairy isolates was decreased, compared with organic dairy isolates. Low proportions of isolates resistant to ampicillin (< 10%) and moderate proportions resistant (30% to 60%) to kanamycin, sulfamethoxazole, and tetracycline were observed on both farm types. The proportion of isolates resistant to tetracycline was higher for conventional than organic farms.

Conclusions and clinical relevance: Campylobacter isolates from dairy cattle and farms managed organically and conventionally had similar patterns of antimicrobial resistance; the proportion of resistant isolates was higher for conventional than organic farms.