Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006 Sep;151(9):1735-48.
doi: 10.1007/s00705-006-0752-9. Epub 2006 Apr 3.

Molecular analysis of Brazilian strains of bovine coronavirus (BCoV) reveals a deletion within the hypervariable region of the S1 subunit of the spike glycoprotein also found in human coronavirus OC43

P E Brandão  1 , F Gregori, L J Richtzenhain, C A R Rosales, L Y B Villarreal, J A Jerez
Affiliations

Molecular analysis of Brazilian strains of bovine coronavirus (BCoV) reveals a deletion within the hypervariable region of the S1 subunit of the spike glycoprotein also found in human coronavirus OC43

P E Brandão et al. Arch Virol. 2006 Sep.

Abstract

Bovine coronavirus (BCoV) causes enteric and respiratory dis- orders in calves and dysentery in cows. In this study, 51 stool samples of calves from 10 Brazilian dairy farms were analysed by an RT-PCR that amplifies a 488-bp fragment of the hypervariable region of the spike glycoprotein gene. Maximum parsimony genealogy with a heuristic algorithm using sequences from 15 field strains studied here and 10 sequences from GenBank and bredavirus as an outgroup virus showed the existence of two major clusters (1 and 2) in this viral species, the Brazilian strains segregating in both of them. The mean nucleotide identity between the 15 Brazilian strains was 98.34%, with a mean amino acid similarity of 98%. Strains from cluster 2 showed a deletion of 6 amino acids inside domain II of the spike protein that was also found in human coronavirus strain OC43, supporting the recent proposal of a zoonotic spill- over of BCoV. These results contribute to the molecular characterization of BCoV, to the prediction of the efficiency of immunogens, and to the definition of molecular markers useful for epidemiologic surveys on coronavirus-caused diseases.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Abraham S, Kienzle TE, Lapps W, Brian DA. Deduced sequence of the bovine coronavirus spike protein and identification of the internal proteolytic cleavage site. Virology. 1990;176:296–301. doi: 10.1016/0042-6822(90)90257-R. - DOI - PMC - PubMed
    1. Akashi H, Inaba Y, Miura Y, Tokuhisha S, Sato K, Satoda K. Properties of a coronavirus isolated from a cow with epizootic diarrhea. Vet Microbiol. 1980;5:265–276. doi: 10.1016/0378-1135(80)90025-5. - DOI
    1. Baric RS, Yount B, Hensley L, Peel SA, Chen W. Episodic evolution mediates interspecies transfer of a murine coronavirus. J Virol. 1997;71:1946–1955. - PMC - PubMed
    1. Benfield DA, Saif LJ. Cell culture propagation of a bovine coronavirus isolated from cows with winter dysentery. J Clin Microbiol. 1990;28:1454–1457. - PMC - PubMed
    1. Bergmann CC, Yao Q, Lin M, Stohlman SA. The JHM strain of mouse hepatitis virus induces a spike protein-specific Db-restricted cytotoxic T cell response. J Gen Virol. 1996;77:315–325. - PubMed

Publication types

MeSH terms

LinkOut - more resources