The 5' terminal structure of the methylated mRNA synthesized in vitro by vesicular stomatitis virus
- PMID: 165900
- DOI: 10.1016/0092-8674(75)90091-4
The 5' terminal structure of the methylated mRNA synthesized in vitro by vesicular stomatitis virus
Abstract
The 5' terminal structure of the mRNA synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus in the presence of S-adenosyl-L-methione consists of 7-methyl guanosine linked to 2'-O-methyl adenosine through a 5'-5' pyrophosphate bond as m7G(5')ppp(5')A-m-p ... The alpha and beta phosphated of GTP and alpha phosphate of ATP are incorporated into the blocked 5' terminal structure.
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