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. 1972 Aug;69(8):2155-9.
doi: 10.1073/pnas.69.8.2155.

Catalytic accelerations of 10-fold by an enzyme-like synthetic polymer

H C Kiefer  1 , W I Congdon, I S Scarpa, I M Klotz
Affiliations

Catalytic accelerations of 10-fold by an enzyme-like synthetic polymer

H C Kiefer et al. Proc Natl Acad Sci U S A. 1972 Aug.

Abstract

A synthetic polymer, polyethyleneimine containing dodecyl (C(12)H(25))-chains for binding sites and imidazole moieties for functional catalytic groups, catalyzes the hydrolysis of phenolic sulfate esters by a two-step mechanism resembling that of the corresponding natural enzyme. Quantitative rate studies give kinetic parameters that show that the polymer (synzyme) accelerates the rate 10(12)-fold compared to unbound imidazole, and 10(2)-fold compared to a type IIA aryl sulfatase enzyme.

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References

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