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. 1972 Dec;69(12):3501-3.
doi: 10.1073/pnas.69.12.3501.

Mutants that produce nitrogenase in the presence of ammonia

J K Gordon  1 , W J Brill
Affiliations

Mutants that produce nitrogenase in the presence of ammonia

J K Gordon et al. Proc Natl Acad Sci U S A. 1972 Dec.

Abstract

Mutants of Azotobacter vinelandii that fix N(2) in the presence of excess NH(4) (+) have been isolated. A mutant that was unable to synthesize component I and component II of nitrogenase was spontaneously reverted to the N(2)-fixing phenotype. Of 21 revertants picked, 7 revertants were not as sensitive as the wild type to repression. A derepressed mutant is as sensitive as the wild type to growth inhibition by 2-methylalanine in the presence of glucose.

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References

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