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. 1973 Mar;70(3):658-60.
doi: 10.1073/pnas.70.3.658.

Biosynthesis of cytokinins in cytokinin-autotrophic tobacco callus

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Biosynthesis of cytokinins in cytokinin-autotrophic tobacco callus

J W Einset et al. Proc Natl Acad Sci U S A. 1973 Mar.

Abstract

A cytokinin-autotrophic strain of tobacco callus contained cytokinin-active compounds with chromatographic mobilities on Sephadex LH-20 corresponding to ribosylzeatin, zeatin, and 6-Delta(2)-isopentenylaminopurine. Zeatin, the apparent major cytokinin, was estimated to be present at a concentration of 10(-4) mumol/kg of tissue. Cytokinin-autotrophic callus supplied with [(14)C]adenine produced radioactive components with the same chromatographic properties as zeatin and 6-Delta(2)-isopentenylaminopurine. These components were not obtained from cytokinin-dependent tissue supplied with [(14)C]adenine in the same manner.

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References

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