Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Dec;73(12):4309-13.
doi: 10.1073/pnas.73.12.4309.

Endonuclease recognition sites mapped on Zea mays chloroplast DNA

J R Bedbrook  1 , L Bogorad
Affiliations

Endonuclease recognition sites mapped on Zea mays chloroplast DNA

J R Bedbrook et al. Proc Natl Acad Sci U S A. 1976 Dec.

Abstract

The closed-circular DNA molecules of 85 x 10(6) daltons from Zea mays chloroplasts were isolated, digested with the restriction endonucleases Sal I, Bam I, and EcoRI, and the resulting fragments sized by agarose gel electrophoresis. A map of maize chloroplast DNA showing the relative location of all the Sal I recognition sequences and many of the Bam I and EcoRI recognition sites was determined. A DNA sequence representing approximately 15% of the Zea mays chloroplast genome is repeated. The two copies of this sequence are in an inverted orientation with respect to one another and are separated by a nonhomologous sequence representing approximately 10% of the genome length. The inverted repeats contain the genes for chloroplast ribosomal RNAs.

PubMed Disclaimer

References

    1. J Mol Biol. 1974 Jan 5;85(4):475-84 - PubMed
    1. Biochim Biophys Acta. 1975 Sep 1;402(3):372-90 - PubMed
    1. J Biol Chem. 1975 Jul 10;250(13):4888-95 - PubMed
    1. J Mol Biol. 1975 Sep 5;97(1):123-5 - PubMed
    1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed

LinkOut - more resources