doi: 10.1073/pnas.75.10.4661.
A photochemical technique for measuring lateral diffusion of spin-labeled phospholipids in membranes
Affiliations
- PMID: 16592571
- PMCID: PMC336176
- DOI: 10.1073/pnas.75.10.4661
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A photochemical technique for measuring lateral diffusion of spin-labeled phospholipids in membranes
Proc Natl Acad Sci U S A.
1978 Oct.
Abstract
A technique is described for the measurement of lateral diffusion of any spin-labeled molecule in planar phospholipid multilayers, using low probe concentrations and a photochemical reaction to generate the initial concentration gradient. Features of the technique include simplicity of data analysis, versatility, and applicability over a wide range of motional rates. Measurements have been made on dimyristoyl phosphatidylcholine and dipalmitoyl phosphatidylcholine in the fluid phase (using a phospholipid with head-group spin label), and excellent agreement is found with recent nuclear magnetic resonance spin-echo measurements, as well as with other spin-label and fluorescence photobleaching results.
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