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. 1975;19(2):65-70.
doi: 10.1159/000458975.

Fluorimetric determination of dipeptidyl carboxypeptidase. (angiotensin-I-converting enzyme)

D Depierre, M Roth

Fluorimetric determination of dipeptidyl carboxypeptidase. (angiotensin-I-converting enzyme)

D Depierre et al. Enzyme. 1975.

Abstract

A fluorimetric assay of dipeptidyl carboxypeptidase is described. It involves incubation at 37 degrees C with the substrate, Z-Phe-His-Leu, and reaction of the dipeptide His-Leu which is released upon enzymatic hydrolysis, with o-phthalaldehyde to yield a fluorescent compound. The method is simple, precise and sensitive. The assay in serum is conveniently performed on 20 mul samples. Normal values in human serum range from 0.04 to 0.22 U/l.

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