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. 1980 Nov;77(11):6448-52.
doi: 10.1073/pnas.77.11.6448.

Recombination between higher plant DNA and the Ti plasmid of Agrobacterium tumefaciens

M F Thomashow  1 , R Nutter, K Postle, M D Chilton, F R Blattner, A Powell, M P Gordon, E W Nester
Affiliations

Recombination between higher plant DNA and the Ti plasmid of Agrobacterium tumefaciens

M F Thomashow et al. Proc Natl Acad Sci U S A. 1980 Nov.

Abstract

The Ti plasmid sequences (T-DNA) from the octopine-producing crown gall tumor A6S/2 were isolated by molecular cloning, using the bacteriophage lambda vector Charon 4A. Analysis of the cloned DNA segments indicates that the Ti plasmid sequences are covalently joined to plant nuclear DNA. These data demonstrate that genetic recombination between a eukaryote and a prokaryote can occur as a natural phenomenon.

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References

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